Oral squamous cell carcinoma (OSCC) is one of the most well-known malignancies that affect the human population worldwide.The early diagnosis and early intervention of OSCC help improve the survival rate of the patients.The tumour free surgical margins are a positive prognostic factor for recurrence-free survival.The molecular markers can be used to detect the tumour free surgical margins.Aim: The aim of the study is to evaluate the expression of p53 & Cyclin D1 marker in resected surgical apparently clear margins here and to correlate the p53 & Cyclin D1 expression with clinicopathological characteristics and patient outcome.
Methods: The study population included retrospective cases of OSCC with apparently clear margins (2017-18) n=10 and Clinicopathological variables relevant to survival analysis were recorded.Finally, two margins were selected from each case, a total of 20 soiebiologique.com margins were included in this study.Paraffin-embedded wax blocks retrieved and tissue sections were made.Expression of cyclin D1 and p 53 was assessed by the immunohistochemical staining procedure Results: Positive expressions Cyclin D1 in 40% of mild dysplasia margins and 60% in clearance adequate margins were present.p53 expression was seen in 16% of mild dysplasia margins and 84% in clearance adequate margins.
The expression of p53 and Cyclin D1 molecular markers are noted in the basal & parabasal layer of epithelium.Conclusion: Molecular markers could play a more reliable method for the assessment of dysplasia at the margins.